An improved procedure for the synthesis of glycine and taurine conjugates of bile acids.
نویسندگان
چکیده
Glycine and taurine conjugates of 5beta-cholanic acids have been synthesized using improved procedures based on the peptide coupling reagent, N-ethoxycarbonyl-2-ethoxy-1,2-dihydroquinoline. The conjugates are obtained in chromatographically pure form in yields higher than 90%. The use of this procedure in the large scale preparation of cholyl[1,2-13C2]glycine is described.
منابع مشابه
The biochemical basis for the conjugation of bile acids with either glycine or taurine.
All animals, except for the placental mammals, conjugate their bile acids exclusively with taurine. However, in certain of the placental mammals, glycine conjugates are also found. The basis for the appearance of glycine conjugation among the placental mammals was investigated. The reaction of choloyl-CoA with glycine and taurine, as catalysed by the soluble fraction from guinea-pig liver, had ...
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Ileal disease, liver disease, and other pathologic conditions have been shown to affect the glycine:taurine ratio (1). The procedure used for determining the glycine:taurine ratio is to separate the individual conjugated bile acids on thin-layer chromatograms and estimate the quantities of each bile acid to get the ratio (2, 3). Sometimes two or more solvent systems are needed to separate the i...
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Fasting and postprandial plasma concentrations of glycine and taurine conjugates of cholic, chenodeoxycholic, and deoxycholic acid were measured by a high pressure liquid chromatography-enzymatic assay in nine healthy subjects. The mean value of each bile acid concentration increased significantly (2.4-4.7 times) in the postprandial period. The total glycine taurine ratio of 2.5 in the fasting ...
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متن کاملQuantitative assay of conjugated and free bile acids as heptafluorobutyrate derivatives by gas-liquid chromatography.
Quantitative analyses of individual bile acids in biological samples are limited by the lengthy multistep preparations necessary. Using heptafluorobutyric acid anhydride in pyridine as derivatizing agent, we reduced several steps to one. Bile acids and their glycine and taurine conjugates form stable heptafluorobutyrate derivatives, climinating the need for deconjugation and preparation of meth...
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ورودعنوان ژورنال:
- Journal of lipid research
دوره 18 3 شماره
صفحات -
تاریخ انتشار 1977